Ash Ketchum is the current Champion. He defeated Gary Oak and the Elite Four for this title some time prior to the events of Pokemon Tower Defense. After Professor Oak's lab is attacked by the Rattata, Gary is sent to look for Ash. After defeating Sabrina in Saffron City, she reveals that Mewthree has kidnapped Ash. Mewtwo claims that his Pikachu is the only key to awakening him. According to Sam at Twitch, Ash is not 10 years old in the Pokemon Tower Defense games.(I ask him if Ash is 10, Sam said he's about 40 years old.) In the timeline of PTD 3, Ash and Misty will have a child(boy or girl) who will become the leader of Team Rocket.
- Mt. Moon 2
- Cerulean Gym 2
- Route 24
- In the Route 24 intro, Ash saves Joey and Maruto from Mewthree by using Tauros's Take Down. Ash sends the two of them to help the Player, facing Mewthree by himself. Ash's Tauros gets knocked out, so he sends out Squirtle, Bulbasaur, and Charizard. Ash tells Charizard to use Seismic Toss, Squirtle to slow Mewthree with Bubble, and Bulbasaur to start charging his Solar Beam. After all of them attack, Mewthree appears out of nowhere saying that they only defeated his Substitute and while they destroyed it he used Recover and has completely healed. Ash and his Pokemon fall victim to Mewthree's Psychic, leaving them vulnerable to Mewthree's Selfdestruct.
- Vermillion Gym
- In the opening cutscene to Vermillion Gym, Mewthree and Ash lie in the same spot as they were left after their battle in Route 24. Mewtwo appears in an attempt to help Ash, but Brock sneaks up behind him with Ash's Master Ball and captures him. Mewthree stands back up, stating that he used an illusion of Ash to lure Mewtwo out of hiding.
|Bulbasaur||Vine Whip, Solar Beam (Not in Battle)|
|Charizard||Flamethrower, Seismic Toss (Not in Battle)|
|Squirtle||Hydro Pump, Bubble (Not in Battle)|
|Ash Ketchum • Gary Oak • Joey • Mewtwo • Maruto|
|Mewthree • James • Jessie • Meowth • Jerry • Tom • Giovanni|
|Brock • Misty • Lt. Surge • Erika • Sabrina • Janine • Blaine • Gary Oak|
|Professor Oak • Camper Luigi • Cal • Bug Catcher • Old Man • Kyogre • Bill • Celebi • Bruno • Sam • Sensei • Groudon • Lorelei • Koga • Lance • Mew|